Daniel Ginsburg, Ph.D.
Title: Professor
Department: Natural Sciences
Office: Loyola Hall 215
Phone: (610) 647-4400 Ext: 3272
Email: dginsburg@immaculata.edu
Education:
Ph.D. in Genetics, Stanford University
B.S. in Chemistry, Stanford University
BIOGRAPHY:
Research Interests:
- Investigating the role of chromatin modifications during transcription elongation with an emphasis on the relationship between histone methylation and acetylation.
- Postdoctoral research, Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health. Determined the role of the NuA4 lysine acetyltransferase complex during transcription elongation in S. cerevisiae. Developed a model for the mechanism of NuA4 recruitment to coding sequences.
- Doctoral research, Stanford University. Developed phage integrases as tools for site-specific integration in mammalian genomes. Examined integrases from phage ΦC31, TP901-1, A118, and ΦBT1 for function in vitro and in mammalian cell culture. Screened mutant attachment site libraries for ΦC31 integrase to improve integration system and determine sequence dependence of ΦC31 recombination reaction. Explored non-DNA sequence factors involved in ΦC31 mediated chromosomal integration.
Publications:
Sathianathan A., Ravichandran P., Lippi J.M., Cohen L., Messina A., Shaju S., Swede M.J. and Ginsburg, D.S. “The Eaf3/5/7 subcomplex stimulates binding of NuA4 to dimethylated H3K36.” J Biol Chem. 2016 Aug 17.
Ginsburg DS, Anlembom TE, Wang J, Patel SR, Li B, Hinnebusch AG. “NuA4 links methylation of histone H3 lysines 4 and 36 to acetylation of histones h3 and H3.” J Biol Chem. 2014 Oct 9. 2014 Nov 21;289(47):32656-70.
Govind C.K., Ginsburg D., Hinnebusch A.G. “Measuring dynamic changes in histone modifications and nucleosome density during activated transcription in budding yeast.” Methods Mol Biol. 2012;833:15-27.
Govind C.K., Qiu H., Ginsburg D.S., Ruan C., Hofmeyer K., Hu C., Swaminathan V., Workman J.L., Li B., Hinnebusch A.G. “Phosphorylated Pol II CTD recruits multiple HDACs, including Rpd3C(S), for methylation-dependent deacetylation of ORF nucleosomes.” Mol Cell. 2010 Jul 30;39(2):234-46.
Ginsburg D.S., Govind, C.K., and Hinnebusch, A.G. “The NuA4 lysine acetyltransferase Esa1 is targeted to coding regions and stimulates transcription elongation with Gcn5.” Mol. Cell Biol. 2009 Dec;29(24):6473-87.
Ginsburg D.S. and Calos M.P. “Site-specific integration with ΦC31 integrase for prolonged expression of therapeutic genes.” Adv Genet. 54:179-87. 2005.
Ginsburg D.S., Thyagarajan, B., Phillips, J. E., and Calos, M. P. “Gene delivery by viruses.” In Encyclopedia of Life Sciences http://www.els.net/ London; Nature Publishing Group. 2004.
Stoll S.M., Ginsburg D.S., Calos M.P. “Phage TP901-1 site-specific integrase functions in human cells.” J Bacteriol. 184(13):3657-63. 2002.
Thyagarajan B., Olivares E.C., Hollis R.P., Ginsburg D.S., and Calos M.P. “Site-specific genomic integration in mammalian cells mediated by phage phiC31 integrase.” Mol. Cell Biol., 21(12):3926-34. 2001.
Presentations:
(Selected)
Bacchus S. and Ginsburg D.S. “Histone H2A I112 and Q114 may stimulate NuA4 binding to nucleosomes.” Metropolitan Association of College and University Biologists, Old Westbury, New York. October 2016.
Butcher N.R. and Ginsburg D.S. “h3 tail mutants reduce h3 acetylation.” Metropolitan Association of College and University Biologists, Old Westbury, New York. October 2016.
Messina A. and Ginsburg D.S. “Set3C stimulates NuA4 interaction with nucleosomes and occupancy at transcribed genes.” Metropolitan Association of College and University Biologists, Old Westbury, New York. October 2016. Awarded first place among graduate students in Developmental Biology and Genetics.
Rose T.E. and Ginsburg D.S. “H3 acetylation affects NuA4-nucleosome interaction.” Metropolitan Association of College and University Biologists, Old Westbury, New York. October 2016. Awarded first place among Master’s students in Developmental Biology and Genetics.
Shaju S. and Ginsburg D.S. “Chromatin Condensation Protects DNA from MMS Damage.” Metropolitan Association of College and University Biologists, Old Westbury, New York. October 2016. Awarded first place among Master’s students in Microbiology and Immunology.
Vashishtha V. and Ginsburg D.S. “Where is the NLS in Tip60?” American Society for Clinical Laboratory Science annual meeting. Philadelphia, PA. August 2016. Awarded best student poster.
Bacchus S., Butcher N.R., Stengel B.T. and Ginsburg D.S. “What parts of the nucleosome does NuA4 bind to?” LIU Post Research and Internship Symposium, Brookville, New York. April 2016.
Davis S.A. and Ginsburg D.S. “Effects of Increased Nuclear Localization of Tip60 in Cancer Cells.” LIU Post Research and Internship Symposium, Brookville, New York. April 2016.
Almish M., Ginsburg D.S. “NuA4 interaction with RNA polymerase II is stimulated by phosphorylation of CTD serines 2 and 5.” Metropolitan Association of College and University Biologists, Montclair, New Jersey. November 2015. Awarded first place among Master’s students in Developmental Biology and Genetics.
Koonvisal M., Ginsburg D.S. “Do NuA4 and Rpd3C(S) compete for binding to chromatin?” Metropolitan Association of College and University Biologists, Montclair, New Jersey. November 2015.
Sathianathan A., Ravichandran P., Lippi J.M., Cohen L., and Ginsburg D.S. “The Eaf3/5/7 subcomplex stimulates NuA4 binding to multiple histone modifications.” Cold Spring Harbor Laboratory Mechanisms of Eukaryotic Transcription Meeting, Cold Spring Harbor, New York. August 2015.
Malatesta C.J. and Ginsburg D.S. “NuA4 and Its Interaction with RNA Polymerase II.” LIU Post Research and Internship Symposium, Brookville, New York. April 2015.
Mathew V. and Ginsburg D.S. “Do NuA4 and Set3C compete for binding to nucleosomes?” LIU Post Research and Internship Symposium, Brookville, New York. April 2015.
Ravichandran P., Ginsburg D.S. “Tip60 Overexpression Exacerbates Chemotherapeutic Drug Treatment in Breast, Pancreatic, and Lung Cancer Cell Lines.” American Society for Biochemistry and Molecular Biology Annual Meeting. Boston, MA. March 2015. Awarded travel award to attend.
Stabile J., Ginsburg D.S. “Chromatin and its role in the protection of DNA.” Metropolitan Association of College and University Biologists, Rockville Centre, New York. November 2014.
Elvuchio T., Ginsburg, D.S. “The effect of h3 mutation on H3 acetylation.” Metropolitan Association of College and University Biologists, Paramus, New Jersey. October 2013.
Wang J., Ginsburg, D.S. “How does H3 methylation affect h3 acetylation in the absence of HDACs?” Metropolitan Association of College and University Biologists, Paramus, New Jersey. October 2013.
Yadamakanti K., Ginsburg, D.S. “Relative contributions of Pol II CTD phosphorylation and histone methylation to NuA4 occupancy in coding sequences.” Metropolitan Association of College and University Biologists, Paramus, New Jersey. October 2013.
Satianathan A., Ravichandran P., Ginsburg D.S. “Eaf3 Stimulates NuA4 Interaction with Methylated H3K36.” Cold Spring Harbor Laboratory Mechanisms of Eukaryotic Transcription Meeting, Cold Spring Harbor, New York. August 2013.
Ginsburg D.S. and Hinnebusch A.G. “NuA4 links H3 methylation to acetylation of histones H3 and h3.” Invited presentation at Montclair State University. Montclair, NJ. April 28, 2011.
Patel S.R., Kollu V.S., Patel D.G., and Ginsburg D.S. “NuA4 binds methylated nucleosomes through multiple subunits.” Cold Spring Harbor Laboratory Mechanisms of Eukaryotic Transcription meeting. 2011.
Ginsburg D.S., Patel S.R., and Hinnebusch A.G. “NuA4 links histone H3 methylation to H3 and h3 acetylation.” Cold Spring Harbor Laboratory Mechanisms of Eukaryotic Transcription meeting. 2011.
Ginsburg D.S., Govind C.K., and Hinnebusch A.G. “The NuA4 lysine acetyltransferase Esa1 is targeted to coding regions and stimulates transcription elongation with Gcn5.” Cold Spring Harbor Laboratory Mechanisms of Eukaryotic Transcription meeting. 2009.
Govind C.K., Hofmeyer K., Ginsburg D.S., and Hinnebusch A.G. “Recruitment of HDACs RPD3S and SET3C (HOS2) Requires Ser5/6-CDT Kinase Kin28 But Not Histone H3 Methylation.” Cold Spring Harbor Laboratory Mechanisms of Eukaryotic Transcription meeting. 2009.
Ginsburg D.S., Govind C.K., and Hinnebusch A.G. “NuA4 Acts As a Positive Elongation Factor Through Stimulation of RSC Recruitment.” American Society of Biochemistry and Molecular Biology Transcriptional Regulation meeting. 2008.
Professional Memberships:
- Council On Undergraduate Research
- Metropolitan Association of College and University Biologists
- American Society for Biochemistry and Molecular Biology
Awards:
2009 Fellows Award for Research Excellence, National Institutes of Health
Dissertation:
Characterization of a site-specific integration system for mammalian cells
Advisor: Michele Calos